true アストリサイト特異的グリア繊維性酸性蛋白質を失損する。 RBRC00408 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Gomi et al., Neuron, 14, 29-41 (1995). C (3-6 months) E14 [129P2/OlaHsd] Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile. 1995年京都大学ウイルス研究所にて五味浩司先生と糸原重美先生によって作製された。 In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Gomi et al., Neuron, 14, 29-41 (1995). 繁殖効率：A 特になし E. coli lacZ, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse glial fibrillary acidic protein genomic DNA C（3〜6か月） ShigeYoshi ITOHARA Gfap KO, GFAP-KO-clone 27 Gfap KO, GFAP-KO-clone 27 Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Developed by Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1995. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed to C57BL/6J. RIKEN,BSI 糸原　重美 Gfap knockout mice. Immediately downstream of the endogenous start codon of Gfap was replaced with a cassette, containing a promoterless lacZ and pgk-neo. Gfap, glial fibrillary acidic protein is an intermediate filament protein specifically expressed in astrocytes. Homozygous mutant mice develop normally and not show any detectable abnormalities in the anatomy of the brain. But, exhibit a impairment of long-term depression (LTD) and PF-Purkinje cell synapses and eyeblink, suggested that the Gfap is required for communications between Bergmann glia and Purkinje cells. <a href='https://brc.riken.jp/mus/pcr00408'>Genotyping protocol -PCR-</a> 繁殖効率：A特になし 理化学研究所脳科学総合研究センター B6.129P2-Gfap<tm1Ito>/ItoRbrc B6.129P2-Gfap<tm1Ito>/ItoRbrc Neurobiology Research